Journal: PLoS ONE

Article Title: Involvement of B2 Receptor in Bradykinin-Induced Proliferation and Proinflammatory Effects in Human Nasal Mucosa-Derived Fibroblasts Isolated from Chronic Rhinosinusitis Patients

doi: 10.1371/journal.pone.0126853

Figure Lengend Snippet: (A) Immunohistochemical staining showing fibroblast distribution in the normal and CRSsNP nasal mucosa. Inferior turbinates (control) and CRSsNP nasal mucosa were stained with anti-fibroblast Ab. Please note that immunoreactivity for fibroblasts was stronger in the submucosal stroma of CRSsNP specimens than in the nasal septal deviation controls. The arrow indicates a positive staining for fibroblast. ep: epithelial cells; v: blood vessels; g: submucosal glands. Scale bar = 100 μm. (B) Immunocytochemical staining of primary cultured NMDFs. Cells grown on chamber slides in subconfluent and confluent conditions were fixed, stained with anti-vimentin Ab, and analyzed under phase-contrast and fluorescence microscopy. This is representative of three similar experiments.

Article Snippet: The sections were then washed in TBS (Tris-buffered saline containing 1% CaCl 2 ), immersed in sodium citrate buffer (pH 6.0), and heated on a water bath for 20 min. After blocking with buffer containing 10% FBS, the slides were incubated at 4 ° C overnight with primary Ab specific for fibroblasts (clone TE-7, mouse monoclonal IgG1, Millipore, Billerica, MA, USA), B1R, B2R (Abcam) or KNG/BK (LifeSpan BioSciences, Inc., Seattle, WA, USA).

Techniques: Immunohistochemical staining, Staining, Control, Cell Culture, Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Involvement of B2 Receptor in Bradykinin-Induced Proliferation and Proinflammatory Effects in Human Nasal Mucosa-Derived Fibroblasts Isolated from Chronic Rhinosinusitis Patients

doi: 10.1371/journal.pone.0126853

Figure Lengend Snippet: NMDFs were treated with the indicated concentrations of BK for 16 h, and (A) cell proliferation was determined by the MTT assay and direct cell counting assay (n = 3–4). (B) The expression of CAMs and COXs was determined by western blotting (n = 4). (C) Effect of BK on monocytes adhesion to fibroblasts. Twenty-four well plates seeded with or without NMDFs were treated with BK (1 μM) for 16 h. Then, BCECF/AM-labeled monocytes were allowed to adhere to the wells for 1 h. After a brief wash, monocytes adhered to wells (green spots) were determined by direct cell counting under the high-powered field (HPF) (n = 4). * P <0.05, ** P <0.01, *** p < 0.001 versus basal (BK 0 μM).

Article Snippet: The sections were then washed in TBS (Tris-buffered saline containing 1% CaCl 2 ), immersed in sodium citrate buffer (pH 6.0), and heated on a water bath for 20 min. After blocking with buffer containing 10% FBS, the slides were incubated at 4 ° C overnight with primary Ab specific for fibroblasts (clone TE-7, mouse monoclonal IgG1, Millipore, Billerica, MA, USA), B1R, B2R (Abcam) or KNG/BK (LifeSpan BioSciences, Inc., Seattle, WA, USA).

Techniques: MTT Assay, Cell Counting, Expressing, Western Blot, Labeling

Journal: Cell metabolism

Article Title: Human skeletal muscle CD90 + fibro-adipogenic progenitors are associated with muscle degeneration in type 2 diabetic patients

doi: 10.1016/j.cmet.2021.10.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TE-7 (mouse) , Merck Millipore , Cat# CBL271, RRID:AB_93449.

Techniques: Recombinant, Blocking Assay, Staining, Imaging, Sample Prep, Software, Flow Cytometry, RNA Sequencing, Quantitative Proteomics

Journal: Cell metabolism

Article Title: Human skeletal muscle CD90 + fibro-adipogenic progenitors are associated with muscle degeneration in type 2 diabetic patients

doi: 10.1016/j.cmet.2021.10.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TE-7 (mouse) , Merck Millipore , Cat# CBL271, RRID:AB_93449.

Techniques: Recombinant, Blocking Assay, Staining, Imaging, Sample Prep, Software, Flow Cytometry, RNA Sequencing, Quantitative Proteomics

Journal: Science Advances

Article Title: Modeling long QT syndrome type 2 on-a-chip via in-depth assessment of isogenic gene-edited 3D cardiac tissues

doi: 10.1126/sciadv.abq6720

Figure Lengend Snippet: ( A to C ) Characterization of CM differentiation of WT and edited hiPSCs, including (A) immunostaining for sarcomeric α-actinin (SAA, green) and CX43 (red), (B) lack of pluripotency marker expression, and (C) expression of CM-specific genes. ( D to F ) Characterization of CF differentiation of WT and edited hiPSCs, including (D) immunostaining for TE7 (green) and vimentin (red), (E) lack of pluripotency marker expression, and (F) expression of fibroblast-specific genes. Scale bars, 50 μm.

Article Snippet: Primary antibodies [mouse anti-SAA (A7811, Sigma-Aldrich), mouse anti-TNNT2 (MA512960, Thermo Fisher Scientific), mouse anti-TE7 (CBL271, Sigma-Aldrich), rabbit anti-connexin43 (ab11370, Abcam), and rabbit anti-hERG (ab196301, Abcam)] were diluted at 1:200 in 10% goat serum and added to the samples for 30 min at 37°C.

Techniques: Immunostaining, Marker, Expressing

Journal: Journal of Visualized Experiments : JoVE

Article Title: Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

doi: 10.3791/52049

Figure Lengend Snippet:

Article Snippet: Name/antigen Antibody species and isotype Clone name Dilution for use Catalogue no. Company Myogenic markers: CD56 (N-CAM) Mouse mc IgG 1 MY-31 (1:100) 347740 BD Desmin Rabbit mc IgG 1 D93F5 (XP) (1:250) 5332S NEB Myogenin Mouse mc IgG 1 F5D (1:50) F5D DSHB Myosin Heavy Chain Mouse mc IgG2b, kappa light chain MF20 (1:200) MF20 DSHB Fibroblast/connective tissue markers: Anti- human fibroblast Mouse mc IgG 1 TE-7 (1:100) CBL271 Millipore PDGFRα Rabbit mc IgG D13C6 (1:500) 5241 NEB Proliferative markers: Ki67 Rabbit mc IgG SP6 (1:200) MP-325-CRM1 MD Adipogenic transcription factors: PPARγ Mouse mc IgG 1 E8 (1:20) Sc-7273 Santa Cruz Biotechnology Key: mc: monoclonal, pc: polyclonal, DSHB: Developmental Studies Hybridoma Bank, Iowa USA; NEB: New England BioLabs, UK; MD: A. Menarini Diagnostics, UK; BD: BD Bioscience, UK.

Techniques:

Journal: Frontiers in Physiology

Article Title: 3D Proximal Tubule Tissues Recapitulate Key Aspects of Renal Physiology to Enable Nephrotoxicity Testing

doi: 10.3389/fphys.2017.00123

Figure Lengend Snippet: List of antibodies used .

Article Snippet: Mouse α-TE7 , 1:500 , EMD Millipore (Temecula, CA).

Techniques: